Anti-Human WASL Polyclonal Antibody (HT262014)

Name: Anti-Human WASL Polyclonal Antibody
Brand: abinScience
SKU: HT262014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT262014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human WASL (Leu21-Arg148).

Target

N-WASP, WASL, Neural Wiskott-Aldrich syndrome protein

Purification

Purified by antigen affinity column.

Accession

O00401

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Actin nucleation-promoting factor WASL is a ~54 kDa protein. Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.

1. Wu, X. et al. (2006) Nature cell biology 8, 756-63. PMID: 16767080

2. Vingadassalom, D. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 6754-9. PMID: 19366662

3. Zhang, J. et al. (2009) The Journal of biological chemistry 284, 21659-69. PMID: 19487689

4. Gaucher, JF. et al. (2012) The Journal of biological chemistry 287, 34646-59. PMID: 22847007

5. Aitio, O. et al. (2012) Structure (London, England : 1993) 20, 1692-703. PMID: 22921828

6. Miki, H. et al. (1998) Nature 391, 93-6. PMID: 9422512

7. Rajabian, T. et al. (2009) Nature cell biology 11, 1212-8. PMID: 19767742

Note

For research use only.

Images

References

Datasheet

Anti-Human WASL Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WASL Polyclonal Antibody (HT262014)

Overview

Images

References

Datasheet

SDS

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