Human WIPI1 Recombinant Protein (N-His) (HD292012)

Name: Human WIPI1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD292012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HD292012
Описание	Recombinant Human WIPI1 Protein, N-His (HD292012) expressed in E. coli, spanning Leu17-Leu360. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q5MNZ9
Длина белка	Leu17-Leu360
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	40.44 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI-1, WIPI1, WIPI49
Фон	WD repeat domain phosphoinositide-interacting protein 1 is a ~48 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. 1. Proikas-Cezanne, T. et al. (2004) Oncogene 23, 9314-25. PMID: 15602573 2. Grotemeier, A. et al. (2010) Cellular signalling 22, 914-25. PMID: 20114074 3. Geeraert, C. et al. (2010) The Journal of biological chemistry 285, 24184-94. PMID: 20484055 4. Itakura, E. et al. (2010) Autophagy 6, 764-76. PMID: 20639694 5. Gaugel, A. et al. (2012) Journal of molecular signaling 7, 16. PMID: 23088497 6. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066 7. Maeda, S. et al. (2019) eLife 8. PMID: 31271352 8. Kojima, W. et al. (2021) Autophagy 17, 2011-2036. PMID: 33499712
Примечание	For research use only

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Даташит

Human WIPI1 Recombinant Protein (N-His).pdf

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