Anti-Human WIPI1 Polyclonal Antibody (HD292014)

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Datasheet
SDS

Overview

Catalog No.

HD292014

Description

Anti-Human WIPI1 Polyclonal Antibody (HD292014) is a rabbit polyclonal antibody detecting WIPI1. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WIPI1 (Leu17-Leu360).

Target

Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI-1, WIPI1, WIPI49

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q5MNZ9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) is a ~48 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane.

1. Proikas-Cezanne, T. et al. (2004) Oncogene 23, 9314-25. PMID: 15602573

2. Grotemeier, A. et al. (2010) Cellular signalling 22, 914-25. PMID: 20114074

3. Geeraert, C. et al. (2010) The Journal of biological chemistry 285, 24184-94. PMID: 20484055

4. Itakura, E. et al. (2010) Autophagy 6, 764-76. PMID: 20639694

5. Gaugel, A. et al. (2012) Journal of molecular signaling 7, 16. PMID: 23088497

6. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066

7. Maeda, S. et al. (2019) eLife 8. PMID: 31271352

8. Kojima, W. et al. (2021) Autophagy 17, 2011-2036. PMID: 33499712

Note

For research use only

Images

References

Datasheet

Anti-Human WIPI1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WIPI1 Polyclonal Antibody (HD292014)

Overview

Images

References

Datasheet

SDS

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