Human WTAP Recombinant Protein (N-His) (HC538012)

Name: Human WTAP Recombinant Protein (N-His)
Brand: abinScience
SKU: HC538012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HC538012
Descripción	Recombinant Human WTAP Protein, N-His (HC538012) expressed in E. coli, spanning Met1-Asp150. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q15007
Longitud de proteína	Met1-Asp150
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	19.81 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP
Fondo	Pre-mRNA-splicing regulator WTAP is a ~44 kDa protein. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. 1. Yue, Y. et al. (2018) Cell discovery 4, 10. PMID: 29507755 2. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 3. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 4. Schwartz, S. et al. (2014) Cell reports 8, 284-96. PMID: 24981863 5. Ortega, A. et al. (2003) The Journal of biological chemistry 278, 3040-7. PMID: 12444081 6. Horiuchi, K. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17278-83. PMID: 17088532 7. Small, TW. et al. (2006) Circulation research 99, 1338-46. PMID: 17095724
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human WTAP Protein, N-His

Referencias

Datasheet

Human WTAP Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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