LASV GPC Recombinant Protein (N-His & C-TwinStrep) (VK453011)

Name: LASV GPC Recombinant Protein (N-His & C-TwinStrep)
Brand: abinScience
SKU: VK453011
Price: 264 USD
Availability: InStock

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Aperçu

Numéro de catalogue	VK453011
Description	Recombinant LASV GPC Protein, N-His & C-TwinStrep (VK453011) expressed in Insect Cells, spanning Met1-Gly424. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification.
Système d'expression	Insect cells
Numéro d'accès	P08669
Longueur de protéine	Met1-Gly424
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Lassa virus (LASV)
Nature	Recombinant
Applications testées	WB
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>80% as determined by SDS-PAGE.
Masse moléculaire prédite	55.19 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Pre-glycoprotein polyprotein GP complex, Pre-GP-C, Stable signal peptide, SSP, Glycoprotein G1, GP1, Glycoprotein G2, GP2, GPC, GP-C
Contexte	Pre-glycoprotein polyprotein GP complex (GPC) is a ~55 kDa protein. Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion. 1. Katz, M. et al. (2022) Nature 603, 174-179. PMID: 35173332 2. Eichler, R. et al. (2003) EMBO reports 4, 1084-8. PMID: 14555961
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant LASV GPC Protein, N-His & C-TwinStrep
Bioactivity

Detects LASV GPC Antibody (VK453063) in indirect ELISAs.
Bioactivity

Detected by Anti-LASV GPC Antibody (12.1F) (Cat No.: VK453063) in indirect ELISA.
Bioactivity

Detected by Anti-LASV GPC Antibody (10.4B) (Cat No.: VK453053) in indirect ELISA.
Bioactivity

Detected by Anti-LASV GPC Antibody (19.7E) (Cat No.: VK453083) in indirect ELISA.
Western blot

Detected by Anti-GPC monoclonal antibody (Catalog No: HC604016)