MERS-CoV ORF4a Recombinant Protein (N-His) (VK073052)

Name: MERS-CoV ORF4a Recombinant Protein (N-His)
Brand: abinScience
SKU: VK073052
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	VK073052
Description	Recombinant MERS-CoV ORF4a Protein, N-His (VK073052) expressed in E. coli, spanning Met1-Asn109. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	K9N4V0
Longueur de protéine	Met1-Asn109
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Middle East respiratory syndrome-related coronavirus (MERS-CoV)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	14.40 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Non-structural protein ORF4a; ORF4a; ORF4a
Contexte	Non-structural protein ORF4a is a ~12 kDa protein. DsRNA-binding protein that plays a role in the inhibition of host innate response. Suppresses host PACT-induced activation of RIGI and MDA5 and thereby circumvents the production of type I interferons. Also prevents the activation of host NF-kappa-B. Inhibits the integrated stress response (ISR) in the infected cell by binding to dsRNA and inhibiting EIF2AK2-mediated phosphorylation of EIF2S1/eIF2-alpha. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication. 1. Niemeyer, D. et al. (2013) Journal of virology 87, 12489-95. PMID: 24027320 2. Yang, Y. et al. (2013) Protein & cell 4, 951-61. PMID: 24318862 3. Siu, KL. et al. (2014) Journal of virology 88, 4866-76. PMID: 24522921 4. Nakagawa, K. et al. (2018) Journal of virology 92. PMID: 30068649
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant MERS-CoV ORF4a Protein, N-His

Reférences

Fiche technique

MERS-CoV ORF4a Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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