Anti-MERS-CoV ORF4a Polyclonal Antibody (VK073054)

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Overview

Catalog No.

VK073054

Description

Anti-MERS-CoV ORF4a Polyclonal Antibody (VK073054) is a rabbit polyclonal antibody detecting Non-structural protein ORF4a; ORF4a; ORF4a in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Middle East respiratory syndrome-related coronavirus (MERS-CoV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mers-cov ORF4a (Met1-Asn109).

Target

Non-structural protein ORF4a; ORF4a; ORF4a

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

K9N4V0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Non-structural protein ORF4a is a ~12 kDa protein. DsRNA-binding protein that plays a role in the inhibition of host innate response. Suppresses host PACT-induced activation of RIGI and MDA5 and thereby circumvents the production of type I interferons. Also prevents the activation of host NF-kappa-B. Inhibits the integrated stress response (ISR) in the infected cell by binding to dsRNA and inhibiting EIF2AK2-mediated phosphorylation of EIF2S1/eIF2-alpha. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication.

1. Niemeyer, D. et al. (2013) Journal of virology 87, 12489-95. PMID: 24027320

2. Yang, Y. et al. (2013) Protein & cell 4, 951-61. PMID: 24318862

3. Siu, KL. et al. (2014) Journal of virology 88, 4866-76. PMID: 24522921

4. Nakagawa, K. et al. (2018) Journal of virology 92. PMID: 30068649

Note

For research use only

Images

References

Datasheet

Anti-MERS-CoV ORF4a Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MERS-CoV ORF4a Polyclonal Antibody (VK073054)

Overview

Images

References

Datasheet

SDS

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