mgpA Recombinant Protein (N-His) (JN127012)

Name: mgpA Recombinant Protein (N-His)
Brand: abinScience
SKU: JN127012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	JN127012
Descripción	Recombinant mgpA Protein, N-His (JN127012) expressed in E. coli, spanning Thr339-Phe764. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P20796
Longitud de proteína	Thr339-Phe764
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mycoplasma genitalium
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	50.46 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Adhesin P1; Attachment protein; Cytadhesin P1; MgPa; mgpA; MG191
Fondo	Adhesin P140 is a ~159 kDa protein. Part of the major adhesin Nap complex which mediates attachment to host ciliated epithelium. This protein and P110 (mgpC) mutually stabilize each other, and are required for formation of the attachment organelle. The P140-P110 Nap complex plays a major role in motility. The attachment organelle is involved in gliding cell motility and adhesion to host cells. One possible human receptor for this protein is peptidyl-prolyl cis-trans isomerase A (cyclophilin A, PPIA). 1. Burgos, R. et al. (2006) Journal of bacteriology 188, 8627-37. PMID: 17028283 2. García-Morales, L. et al. (2016) Molecular microbiology 100, 125-38. PMID: 26712501 3. Seybert, A. et al. (2018) Molecular microbiology 108, 319-329. PMID: 29470847 4. Deng, X. et al. (2018) International journal of medical microbiology : IJMM 308, 405-412. PMID: 29551599 5. Li, L. et al. (2020) Frontiers in immunology 11, 2052. PMID: 33013867
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant mgpA Protein, N-His

Referencias

Datasheet

mgpA Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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