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Anti-mgpA Polyclonal Antibody (JN127014)

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Overview
Catalog No.JN127014
Description
Anti-mgpA Polyclonal Antibody (JN127014) is a rabbit polyclonal antibody detecting Adhesin P1; Attachment protein; Cytadhesin P1; MgPa; mgpA; MG191 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMycoplasma genitalium
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mgpA (Thr339-Phe764).
Target Adhesin P1; Attachment protein; Cytadhesin P1; MgPa; mgpA; MG191
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P20796
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adhesin P140 (mgpA) is a ~159 kDa protein. Part of the major adhesin Nap complex which mediates attachment to host ciliated epithelium. This protein and P110 (mgpC) mutually stabilize each other, and are required for formation of the attachment organelle. The P140-P110 Nap complex plays a major role in motility. The attachment organelle is involved in gliding cell motility and adhesion to host cells. One possible human receptor for this protein is peptidyl-prolyl cis-trans isomerase A (cyclophilin A, PPIA).

1. Burgos, R. et al. (2006) Journal of bacteriology 188, 8627-37. PMID: 17028283
2. García-Morales, L. et al. (2016) Molecular microbiology 100, 125-38. PMID: 26712501
3. Seybert, A. et al. (2018) Molecular microbiology 108, 319-329. PMID: 29470847
4. Deng, X. et al. (2018) International journal of medical microbiology : IJMM 308, 405-412. PMID: 29551599
5. Li, L. et al. (2020) Frontiers in immunology 11, 2052. PMID: 33013867
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-mgpA Polyclonal Antibody

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