mgpA Recombinant Protein (N-His) (JN127012)

Name: mgpA Recombinant Protein (N-His)
Brand: abinScience
SKU: JN127012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN127012
Description	Recombinant mgpA Protein, N-His (JN127012) expressed in E. coli, spanning Thr339-Phe764. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P20796
Protein length	Thr339-Phe764
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mycoplasma genitalium
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	50.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Adhesin P1; Attachment protein; Cytadhesin P1; MgPa; mgpA; MG191
Background	Adhesin P140 is a ~159 kDa protein. Part of the major adhesin Nap complex which mediates attachment to host ciliated epithelium. This protein and P110 (mgpC) mutually stabilize each other, and are required for formation of the attachment organelle. The P140-P110 Nap complex plays a major role in motility. The attachment organelle is involved in gliding cell motility and adhesion to host cells. One possible human receptor for this protein is peptidyl-prolyl cis-trans isomerase A (cyclophilin A, PPIA). 1. Burgos, R. et al. (2006) Journal of bacteriology 188, 8627-37. PMID: 17028283 2. García-Morales, L. et al. (2016) Molecular microbiology 100, 125-38. PMID: 26712501 3. Seybert, A. et al. (2018) Molecular microbiology 108, 319-329. PMID: 29470847 4. Deng, X. et al. (2018) International journal of medical microbiology : IJMM 308, 405-412. PMID: 29551599 5. Li, L. et al. (2020) Frontiers in immunology 11, 2052. PMID: 33013867
Note	For research use only

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SDS-PAGE for Recombinant mgpA Protein, N-His

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Datasheet

mgpA Recombinant Protein (N-His).pdf

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mgpA Recombinant Protein (N-His) (JN127012)

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