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| カタログ番号 | MW474012 |
|---|---|
| 説明 |
Recombinant Mouse CASP4/Caspase-4 Protein, N-His (MW474012) expressed in E. coli, spanning Pro81-Glu266. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P70343 |
| タンパク質長 | Pro81-Glu266 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 23.42 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Caspase-4, CASP-4, 3.4.22.64, Caspase-11, CASP-11, Protease ICH-3, Caspase-4 subunit p10, Caspase-4 subunit p20, Casp4, Casp11, Caspl, Ich3 |
| 背景 | Caspase-4 is a ~42 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD. In contrast to its human ortholog, does not cleave IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18. 1. Kayagaki, N. et al. (2011) Nature 479, 117-21. PMID: 22002608 2. Aachoui, Y. et al. (2013) Science (New York, N.Y.) 339, 975-8. PMID: 23348507 3. Kayagaki, N. et al. (2013) Science (New York, N.Y.) 341, 1246-9. PMID: 23887873 4. Hagar, JA. et al. (2013) Science (New York, N.Y.) 341, 1250-3. PMID: 24031018 5. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034 6. Lee, BL. et al. (2018) The Journal of experimental medicine 215, 2279-2288. PMID: 30135078 7. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519 8. Wei, C. et al. (2024) Nature 629, 893-900. PMID: 38632402 9. Aachoui, Y. et al. (2015) Cell host & microbe 18, 320-32. PMID: 26320999 10. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Mouse CASP4/Caspase-4 Protein, N-His
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