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Mouse CD218a/IL18R1 Recombinant Protein (N-His) (MC691012)

Mouse CD218a/IL18R1 Recombinant Protein (N-His)
Mouse CD218a/IL18R1 Recombinant Protein (N-His)
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Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Datasheet

  • SDS

Vista general
Número de catálogoMC691012
Descripción
Recombinant Mouse CD218a/IL18R1 Protein, N-His (MC691012) expressed in E. coli, spanning Lys20-Lys314. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ61098
Longitud de proteínaLys20-Lys314
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesMus musculus (Mouse)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 36.40 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosInterleukin-18 receptor alpha, Interleukin-18 receptor 1, IL-18R1, Il18r1, IL1 receptor-related protein, CD218 antigen-like family member A, IL-18R-alpha, IL-18Ralpha, IL1R-rp, CD218a, IL-1Rrp, IL-18R-1
Fondo

Interleukin-18 receptor 1 (CD218a/IL18R1) is a ~61 kDa protein. Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells. Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3.

1. Wang, J. et al. (2015) Nature medicine 21, 820-6. PMID: 26099046
Nota For research use only.
Imágenes
  • Mouse CD218a/IL18R1 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse CD218a/IL18R1 Protein, N-His

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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