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| カタログ番号 | MW630021 |
|---|---|
| 説明 |
Recombinant Mouse CD80/B7-1 Protein, C-His-GPI (MW630021) expressed in Mammalian Cells, spanning Val38-Asn246. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | Q00609 |
| タンパク質長 | Val38-Asn246 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 34.70 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1 |
| 背景 | T-lymphocyte activation antigen CD80 (B7-1) is a ~34 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. 1. Tseng, SY. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 7829-36. PMID: 16339518 2. Freeman, GJ. et al. (1991) The Journal of experimental medicine 174, 625-31. PMID: 1714935 3. Good-Jacobson, KL. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 188, 4217-25. PMID: 22450810 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Mouse CD80/B7-1 Protein, C-His-GPI
Immobilized MW630021 Mouse CD80/B7-1 Recombinant Protein (C-His-GPI) at 2 μg/mL(100 μL/well) can bind HB651021 Human CD152/CTLA4 Recombinant Protein (C-Fc), the EC 50 is 46.0 ng/mL.
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