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Mouse CD8 alpha Recombinant Protein (C-His) (MF815011)

Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Mouse CD8 alpha Recombinant Protein (C-His)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
Contáctenos
  • Vista general

  • Imágenes

  • Referencias

  • Datasheet

  • SDS

Vista general
Número de catálogoMF815011
Descripción
Recombinant Mouse CD8A Protein, C-His (MF815011) expressed in Mammalian Cells, spanning Lys28-Tyr196. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónMammalian cells
Número de accesoP01731
Longitud de proteínaLys28-Tyr196
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesMus musculus (Mouse)
Naturaleza Recombinant
Objetivo CD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 21.78 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosCD8a, Cd8a, Lyt-2, Lyt2, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2
Fondo

T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~27 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Nota For research use only.
Imágenes
  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detects Mouse CD8a/Lyt2 Antibody (YTS105.18) in indirect ELISAs.

  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detects Mouse CD8a/Lyt2 Antibody (2.43) in indirect ELISAs.

  • Mouse CD8 alpha Recombinant Protein (C-His)

    Bioactivity

    Detected by Anti-Mouse CD8a/Lyt2 Antibody (53-6.72) (Catalog No: MF815607) in indirect ELISA

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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