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| カタログ番号 | MS720012 |
|---|---|
| 説明 |
Recombinant Mouse GFRAL Protein, N-GST & C-His (MS720012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6SJE0 |
| タンパク質長 | Met128-Asn318 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | GDNF family receptor alpha-like, Gfral, Gral |
| 背景 | GDNF family receptor alpha-like is a ~43 kDa protein. Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. 1. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097 2. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098 3. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099 4. Hsu, JY. et al. (2017) Nature 550, 255-259. PMID: 28953886 5. Tsai, VW. et al. (2019) International journal of obesity (2005) 43, 2370-2380. PMID: 31152154 6. Borner, T. et al. (2020) Cell metabolism 31, 351-362.e5. PMID: 31928886 7. Worth, AA. et al. (2020) eLife 9. PMID: 32723474 8. Sabatini, PV. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 33593916 9. Lu, JF. et al. (2022) PLoS biology 20, e3001517. PMID: 35202387 10. Benichou, O. et al. (2023) Cell metabolism 35, 274-286.e10. PMID: 36630958 |
| 注意事項 | For research use only |
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