Mouse IFN-alpha/beta R1 Recombinant Protein (N-His) (MB823012)

Name: Mouse IFN-alpha/beta R1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MB823012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	MB823012
Description	Recombinant Mouse IFNAR1 Protein, N-His (MB823012) expressed in E. coli, spanning Asp36-Ile432. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P33896
Longueur de protéine	Asp36-Ile432
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Mus musculus (Mouse)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	47.43 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Ifnar1, Ifar, IFN-alpha/beta receptor 1, IFN-R-1, Type I interferon receptor 1, Ifnar, Interferon alpha/beta receptor 1
Contexte	Interferon alpha/beta receptor 1 (IFNAR1) is a ~65 kDa protein. Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway. 1. Kumar, KG. et al. (2003) The EMBO journal 22, 5480-90. PMID: 14532120 2. Uzé, G. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 4774-8. PMID: 1533935 3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985 4. de Weerd, NA. et al. (2013) Nature immunology 14, 901-7. PMID: 23872679
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Mouse IFNAR1 Protein, N-His
Bioactivity

Detected by MAR1-5A3 (Catalog No: MB823107) in indirect ELISA

Reférences

Fiche technique

Mouse IFN-alpha/beta R1 Recombinant Protein (N-His).pdf

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