

| Каталожный номер | MD457012 |
|---|---|
| Описание |
Recombinant Mouse PM20D1 Protein, N-His (MD457012) expressed in E. coli, spanning Lys233-Leu503. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | Q8C165 |
| Длина белка | Lys233-Leu503 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Mus musculus (Mouse) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 32.52 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | EC:3.5.1.114, EC:3.5.1.14, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, PM20D1, Peptidase M20 domain-containing protein 1 |
| Фон | N-fatty-acyl-amino acid synthase/hydrolase PM20D1 is a ~55 kDa protein. Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles. 1. Long, JZ. et al. (2016) Cell 166, 424-435. PMID: 27374330 2. Lin, H. et al. (2018) Journal of medicinal chemistry 61, 3224-3230. PMID: 29533650 3. Long, JZ. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6937-E6945. PMID: 29967167 4. Kim, JT. et al. (2020) eLife 9. PMID: 32271712 5. Kim, JT. et al. (2020) Cell chemical biology 27, 1130-1139.e4. PMID: 32402239 |
| Примечание | For research use only |
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