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Anti-Mouse PM20D1 Polyclonal Antibody (MD457014)

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Anti-Mouse PM20D1 Polyclonal Antibody
Anti-Mouse PM20D1 Polyclonal Antibody
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Overview
Catalog No.MD457014
Description
Anti-Mouse PM20D1 Polyclonal Antibody (MD457014) is a rabbit polyclonal antibody detecting PM20D1. Suitable for Mouse.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse PM20D1 (Lys233-Leu503).
Target EC:3.5.1.114, EC:3.5.1.14, N-fatty-acyl-amino acid synthase/hydrolase PM20D1, PM20D1, Peptidase M20 domain-containing protein 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q8C165
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

N-fatty-acyl-amino acid synthase/hydrolase PM20D1 is a ~55 kDa protein. Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction (Probable). Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

1. Long, JZ. et al. (2016) Cell 166, 424-435. PMID: 27374330
2. Lin, H. et al. (2018) Journal of medicinal chemistry 61, 3224-3230. PMID: 29533650
3. Long, JZ. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E6937-E6945. PMID: 29967167
4. Kim, JT. et al. (2020) eLife 9. PMID: 32271712
5. Kim, JT. et al. (2020) Cell chemical biology 27, 1130-1139.e4. PMID: 32402239
Note For research use only
Images
  • Anti-Mouse PM20D1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PM20D1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse PM20D1 Polyclonal Antibody

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