ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Mus musculus (Mouse)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
33.82 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG
Фон
Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses.
1. Grimaldi, B. et al. (2010) Cell metabolism 12, 509-20. PMID: 21035761
2. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231
3. Tontonoz, P. et al. (1994) Nucleic acids research 22, 5628-34. PMID: 7838715
4. Tontonoz, P. et al. (1994) Genes & development 8, 1224-34. PMID: 7926726
5. Kliewer, SA. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7355-9. PMID: 8041794
6. Zhu, Y. et al. (1993) The Journal of biological chemistry 268, 26817-20. PMID: 8262913
7. Hinds, TD. et al. (2011) The Journal of biological chemistry 286, 42911-22. PMID: 21994940
8. Lee, KW. et al. (2016) Experimental & molecular medicine 48, e234. PMID: 27197753
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Mouse PPARG Protein, N-His
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