ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
56.61 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG
Background
Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses.
1. Grimaldi, B. et al. (2010) Cell metabolism 12, 509-20. PMID: 21035761
2. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231
3. Tontonoz, P. et al. (1994) Nucleic acids research 22, 5628-34. PMID: 7838715
4. Tontonoz, P. et al. (1994) Genes & development 8, 1224-34. PMID: 7926726
5. Kliewer, SA. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7355-9. PMID: 8041794
6. Zhu, Y. et al. (1993) The Journal of biological chemistry 268, 26817-20. PMID: 8262913
7. Hinds, TD. et al. (2011) The Journal of biological chemistry 286, 42911-22. PMID: 21994940
8. Lee, KW. et al. (2016) Experimental & molecular medicine 48, e234. PMID: 27197753
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Mouse PPARG Recombinant Protein (N-His)
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