●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión
Mammalian cells
Número de acceso
P63089
Longitud de proteína
Gly33-Lys148
Aplicaciones
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Mus musculus (Mouse)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>95% as determined by SDS-PAGE.
Peso molecular predicho
43.13 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Pleiotrophin is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK or AFAP1L2 in order to activate the PI3K-AKT pathway.
1. Hienola, A. et al. (2004) Molecular and cellular neurosciences 26, 75-88. PMID: 15121180
2. Imai, S. et al. (2009) Bone 44, 785-94. PMID: 19442624
3. Kuboyama, K. et al. (2016) The Journal of biological chemistry 291, 18117-28. PMID: 27445335
4. Tang, C. et al. (2019) Neuron 101, 32-44.e6. PMID: 30497772
5. Amet, LE. et al. (2001) Molecular and cellular neurosciences 17, 1014-24. PMID: 11414790
6. Krellman, JW. et al. (2014) PloS one 9, e100597. PMID: 25000129
Nota
For research use only
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Mouse PTN/Pleiotrophin Protein, N-Fc
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