Anti-Mouse PTN/Pleiotrophin Polyclonal Antibody (MA498014)

Name: Anti-Mouse PTN/Pleiotrophin Polyclonal Antibody
Brand: abinScience
SKU: MA498014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

MA498014

Description

Anti-Mouse PTN/Pleiotrophin Polyclonal Antibody (MA498014) is a rabbit polyclonal antibody detecting PTN/Pleiotrophin in ELISA, IHC, WB. Suitable for Mus musculus, Human, Xenopus laevis, Sus scrofa, and Gallus gallus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse PTN/Pleiotrophin (Gly60-Gly142).

Target

PTN/Pleiotrophin

Purification

Purified by antigen affinity column.

Accession

P63089

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Pleiotrophin, PTN, Heparin-binding brain mitogen, HBBM, Heparin-binding growth factor 8, HBGF-8, Heparin-binding growth-associated molecule, HB-GAM, Heparin-binding neutrophic factor, HBNF, Osteoblast-specific factor 1, OSF-1, Ptn

Background

Pleiotrophin (PTN) is a ~18 kDa protein. Secreted growth factor that mediates its signal through cell-surface proteoglycan and non-proteoglycan receptors. Binds cell-surface proteoglycan receptor via their chondroitin sulfate (CS) groups. Thereby regulates many processes like cell proliferation, cell survival, cell growth, cell differentiation and cell migration in several tissues namely neuron and bone. Also plays a role in synaptic plasticity and learning-related behavior by inhibiting long-term synaptic potentiation. Binds PTPRZ1, leading to neutralization of the negative charges of the CS chains of PTPRZ1, inducing PTPRZ1 clustering, thereby causing the dimerization and inactivation of its phosphatase activity leading to increased tyrosine phosphorylation of each of the PTPRZ1 substrates like ALK or AFAP1L2 in order to activate the PI3K-AKT pathway.

1. Hienola, A. et al. (2004) Molecular and cellular neurosciences 26, 75-88. PMID: 15121180

2. Imai, S. et al. (2009) Bone 44, 785-94. PMID: 19442624

3. Kuboyama, K. et al. (2016) The Journal of biological chemistry 291, 18117-28. PMID: 27445335

4. Tang, C. et al. (2019) Neuron 101, 32-44.e6. PMID: 30497772

5. Amet, LE. et al. (2001) Molecular and cellular neurosciences 17, 1014-24. PMID: 11414790

6. Krellman, JW. et al. (2014) PloS one 9, e100597. PMID: 25000129

Note

For research use only.

Images

References

Datasheet

Anti-Mouse PTN/Pleiotrophin Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse PTN/Pleiotrophin Polyclonal Antibody (MA498014)

Overview

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References

Datasheet

SDS

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