Mouse SEMA4A Recombinant Protein (C-Fc) (MA988011)

Name: Mouse SEMA4A Recombinant Protein (C-Fc)
Brand: abinScience
SKU: MA988011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	MA988011
Описание	Recombinant Mouse SEMA4A Protein, C-Fc (MA988011) expressed in Mammalian Cells, spanning Thr33-His682. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	Mammalian cells
Номер доступа	Q62178
Длина белка	Thr33-His682
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Mus musculus (Mouse)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	100.01 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Semaphorin-4A, Semaphorin-B, Sema B, Sema4a, Semab, SemB
Фон	Semaphorin-4A is a ~83 kDa protein. Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Regulates glutamatergic and GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. 1. Toyofuku, T. et al. (2007) The EMBO journal 26, 1373-84. PMID: 17318185 2. Yukawa, K. et al. (2010) International journal of molecular medicine 25, 225-30. PMID: 20043131 3. McDermott, JE. et al. (2018) Molecular and cellular neurosciences 92, 50-66. PMID: 29981480 4. Kumanogoh, A. et al. (2005) Immunity 22, 305-16. PMID: 15780988 5. Kumanogoh, A. et al. (2002) Nature 419, 629-33. PMID: 12374982
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Mouse SEMA4A Protein, C-Fc

Ссылки

Даташит

Mouse SEMA4A Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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