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| カタログ番号 | MA888012 |
|---|---|
| 説明 |
Recombinant Mouse SPNS2 Protein, N-His-KSI (MA888012) expressed in E. coli, spanning Met2-Ala140. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q91VM4 |
| タンパク質長 | Met2-Ala140 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.56 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Spinster homolog 2; Spns2; Spns2 |
| 背景 | Sphingosine-1-phosphate transporter SPNS2 is a ~58 kDa protein. Lipid transporter that specifically mediates export of sphingosine-1-phosphate (sphing-4-enine 1-phosphate, S1P) and sphinganine-1-phosphate in the lymph, thereby playing a role in lymphocyte trafficking. S1P is a bioactive signaling molecule that regulates many physiological processes important for the development and for the immune system. Regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking. Required for the egress of T-cells from lymph nodes during an immune response by mediating S1P secretion, which generates a gradient that enables activated T-cells to access lymph. Also required for the egress of immature B-cells from the bone marrow. 1. Fukuhara, S. et al. (2012) The Journal of clinical investigation 122, 1416-26. PMID: 22406534 2. Nijnik, A. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 102-11. PMID: 22664872 3. Mendoza, A. et al. (2012) Cell reports 2, 1104-10. PMID: 23103166 4. Nagahashi, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1001-11. PMID: 23180825 5. Okuniewska, M. et al. (2021) Cell reports 36, 109368. PMID: 34260944 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant Mouse SPNS2 Protein, N-His-KSI
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