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Anti-Mouse SPNS2 Polyclonal Antibody (MA888014)

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Overview
Catalog No.MA888014
Description
Anti-Mouse SPNS2 Polyclonal Antibody (MA888014) is a rabbit polyclonal antibody detecting Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Spinster homolog 2; Spns2; Spns2 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse SPNS2 (Met2-Ala140).
Target Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Spinster homolog 2; Spns2; Spns2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q91VM4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sphingosine-1-phosphate transporter SPNS2 is a ~58 kDa protein. Lipid transporter that specifically mediates export of sphingosine-1-phosphate (sphing-4-enine 1-phosphate, S1P) and sphinganine-1-phosphate in the lymph, thereby playing a role in lymphocyte trafficking. S1P is a bioactive signaling molecule that regulates many physiological processes important for the development and for the immune system. Regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking. Required for the egress of T-cells from lymph nodes during an immune response by mediating S1P secretion, which generates a gradient that enables activated T-cells to access lymph. Also required for the egress of immature B-cells from the bone marrow.

1. Fukuhara, S. et al. (2012) The Journal of clinical investigation 122, 1416-26. PMID: 22406534
2. Nijnik, A. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 102-11. PMID: 22664872
3. Mendoza, A. et al. (2012) Cell reports 2, 1104-10. PMID: 23103166
4. Nagahashi, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1001-11. PMID: 23180825
5. Okuniewska, M. et al. (2021) Cell reports 36, 109368. PMID: 34260944
Note For research use only
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Formula
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Anti-Mouse SPNS2 Polyclonal Antibody

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