Mouse SPNS2 Recombinant Protein (N-His-KSI) (MA888012)

Name: Mouse SPNS2 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: MA888012
Price: 211 USD
Availability: InStock

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Catalog No.	MA888012
Description	Recombinant Mouse SPNS2 Protein, N-His-KSI (MA888012) expressed in E. coli, spanning Met2-Ala140. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q91VM4
Protein length	Met2-Ala140
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.56 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Spinster homolog 2; Spns2; Spns2
Background	Sphingosine-1-phosphate transporter SPNS2 is a ~58 kDa protein. Lipid transporter that specifically mediates export of sphingosine-1-phosphate (sphing-4-enine 1-phosphate, S1P) and sphinganine-1-phosphate in the lymph, thereby playing a role in lymphocyte trafficking. S1P is a bioactive signaling molecule that regulates many physiological processes important for the development and for the immune system. Regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking. Required for the egress of T-cells from lymph nodes during an immune response by mediating S1P secretion, which generates a gradient that enables activated T-cells to access lymph. Also required for the egress of immature B-cells from the bone marrow. 1. Fukuhara, S. et al. (2012) The Journal of clinical investigation 122, 1416-26. PMID: 22406534 2. Nijnik, A. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 102-11. PMID: 22664872 3. Mendoza, A. et al. (2012) Cell reports 2, 1104-10. PMID: 23103166 4. Nagahashi, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1001-11. PMID: 23180825 5. Okuniewska, M. et al. (2021) Cell reports 36, 109368. PMID: 34260944
Note	For research use only

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SDS-PAGE for Recombinant Mouse SPNS2 Protein, N-His-KSI

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Datasheet

Mouse SPNS2 Recombinant Protein (N-His-KSI).pdf

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Mouse SPNS2 Recombinant Protein (N-His-KSI) (MA888012)

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