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Mouse Timd2/G9D3 protein Recombinant Protein (N-GST & C-His) (MA149012)

Mouse Timd2/G9D3 protein Recombinant Protein (N-GST & C-His)
Mouse Timd2/G9D3 protein Recombinant Protein (N-GST & C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерMA149012
Описание
Recombinant Mouse Timd2/G9D3 protein Protein, N-GST & C-His (MA149012) expressed in E. coli, spanning Ser21-Glu129. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ8R183
Длина белкаSer21-Glu129
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыMus musculus (Mouse)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 39.92 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияG9D3 protein, Timd2
Фон

T-cell immunoglobulin and mucin domain-containing protein 2 (Timd2/G9D3 protein) is a ~33 kDa protein. Cell surface glycoprotein that participates in iron homeostasis in the liver, the kidney, the retina and oligodendrocytes by acting as a receptor of H-ferritin. Mechanistically, mediates iron-containing ferritin uptake via an endocytic pathway, trafficking to endosomes and subsequently to lysosomes. Plays also an important role in the regulation of Th2 immunity. Receptor for SEMA4A involved in the regulation of T-cell function, enhancing T-cell activation.

1. Chen, TT. et al. (2005) The Journal of experimental medicine 202, 955-65. PMID: 16203866
2. Valença, A. et al. (2021) Experimental eye research 202, 108292. PMID: 33065090
3. Chakravarti, S. et al. (2005) The Journal of experimental medicine 202, 437-44. PMID: 16043519
4. Han, J. et al. (2011) PloS one 6, e23800. PMID: 21886823
5. Kumanogoh, A. et al. (2002) Nature 419, 629-33. PMID: 12374982
Примечание For research use only.
Изображения
  • Mouse Timd2/G9D3 protein Recombinant Protein (N-GST & C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse Timd2/G9D3 protein Protein, N-GST & C-His

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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