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| カタログ番号 | JN856012 |
|---|---|
| 説明 |
Recombinant Protein A/SPA Protein, C-His (JN856012) expressed in E. coli, spanning Ala37-Lys327. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P02976 |
| タンパク質長 | Ala37-Lys327 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Staphylococcus aureus |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.88 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from PBS pH 7.4, 1 mM EDTA, 4% trehalose, 1% mannitol データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA, spa, Protein A |
| 背景 | Immunoglobulin G-binding protein A (SPA) is a ~56 kDa protein. Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. 1. Graille, M. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 5399-404. PMID: 10805799 2. Moks, T. et al. (1986) European journal of biochemistry 156, 637-43. PMID: 2938951 3. Forsgren, A. et al. (1966) Journal of immunology (Baltimore, Md. : 1950) 97, 822-7. PMID: 4163007 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Protein A/SPA Protein, C-His
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