Anti-Staphylococcal protein A/SpA Recombinant Antibody (G3) (JN961013)

Name: Anti-Staphylococcal protein A/SpA Recombinant Antibody (G3)
Brand: abinScience
SKU: JN961013
Price: 191 USD
Availability: InStock

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1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN961013

Species reactivity

Staphylococcus aureus

Applications

ELISA

Host species

Human

Isotype

IgG3, Kappa

Clone ID

Clonality

Monoclonal

Target

Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02976

RRID

Anti-Staphylococcal protein A/SpA Antibody (G3) (abinScience Cat# JN961013, RRID:AB_3727790)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Immunoglobulin G-binding protein A (SpA) is a ~56 kDa protein. Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin.

1. Graille, M. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 5399-404. PMID: 10805799

2. Moks, T. et al. (1986) European journal of biochemistry 156, 637-43. PMID: 2938951

3. Forsgren, A. et al. (1966) Journal of immunology (Baltimore, Md. : 1950) 97, 822-7. PMID: 4163007

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcal protein A/SpA Antibody (G3)

References

Datasheet

Anti-Staphylococcal protein A/SpA Recombinant Antibody (G3).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcal protein A/SpA Recombinant Antibody (G3) (JN961013)

Overview

Images

References

Datasheet

SDS

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