Protein A/SPA Recombinant Protein (C-His) (JN856012)

Name: Protein A/SPA Recombinant Protein (C-His)
Brand: abinScience
SKU: JN856012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN856012
Description	Recombinant Protein A/SPA Protein, C-His (JN856012) expressed in E. coli, spanning Ala37-Lys327. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P02976
Protein length	Ala37-Lys327
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Staphylococcus aureus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.88 kDa
Form	Lyophilized
Storage buffer	Lyophilized from PBS pH 7.4, 1 mM EDTA, 4% trehalose, 1% mannitol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA, spa, Protein A
Background	Immunoglobulin G-binding protein A (SPA) is a ~56 kDa protein. Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins. In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. 1. Graille, M. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 5399-404. PMID: 10805799 2. Moks, T. et al. (1986) European journal of biochemistry 156, 637-43. PMID: 2938951 3. Forsgren, A. et al. (1966) Journal of immunology (Baltimore, Md. : 1950) 97, 822-7. PMID: 4163007
Note	For research use only.