SARS-CoV-2 M Recombinant Protein (N-His) (VK795012)

Name: SARS-CoV-2 M Recombinant Protein (N-His)
Brand: abinScience
SKU: VK795012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	VK795012
Description	Recombinant SARS-CoV-2 M Protein, N-His (VK795012) expressed in E. coli, spanning Arg101-Gln222. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P0DTC5
Longueur de protéine	Arg101-Gln222
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	15.87 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Membrane protein, M, E1 glycoprotein, Matrix glycoprotein, Membrane glycoprotein, M Protein
Contexte	Membrane protein (M Protein) is a ~25 kDa protein. Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins. Regulates the localization of S protein at cis-Golgi, the place of virus budding. May act by binding cytoplasmic c-terminus of S. 1. Boson, B. et al. (2021) The Journal of biological chemistry 296, 100111. PMID: 33229438
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant SARS-CoV-2 M Protein, N-His.

Reférences

Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity

Fiche technique

SARS-CoV-2 M Recombinant Protein (N-His).pdf

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