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HEK293T SARS-CoV-2 Membrane Protein (M) Stable Cell Line (VK795818)

HEK293T SARS-CoV-2 Membrane Protein (M) Stable Cell Line
HEK293T SARS-CoV-2 Membrane Protein (M) Stable Cell Line
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Overview
Catalog No.VK795818
Description
HEK293T SARS-CoV-2 Membrane Protein (M) Stable Cell Line (VK795818) is a stable cell line expressing SARS-CoV-2 Membrane Protein (M). Engineered for consistent, high-level target expression in research applications.
Highlights
  • Stable Expression — Engineered for consistent, high-level target expression.
AccessionP0DTC5
ApplicationsFCM
SpeciesViral (SARS-CoV-2)
Host species HEK293T
Target SARS-CoV-2 Membrane Protein (M)
Properties Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage Frozen in liquid nitrogen.
Specifications 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background

Membrane protein (SARS-CoV-2 Membrane Protein (M)) is a ~25 kDa protein. Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins. Regulates the localization of S protein at cis-Golgi, the place of virus budding. May act by binding cytoplasmic c-terminus of S.

1. Boson, B. et al. (2021) The Journal of biological chemistry 296, 100111. PMID: 33229438
Images
  • HEK293T SARS-CoV-2 Membrane Protein (M) Stable Cell Line

    qPCR

    Untransfected cells and Transfected cells were tested with qPCR.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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