

| Numéro de catalogue | JN977012 |
|---|---|
| Description |
Recombinant SUMO tag Control Protein (N-terminal) (JN977012) expressed in E. coli, spanning Met1-Gly98. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q12306 |
| Longueur de protéine | Met1-Gly98 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Saccharomyces cerevisiae (Baker's yeast) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | SUMO-tag Protein Control, SUMO-tag Protein |
| Contexte | Small ubiquitin-related modifier (SUMO-tag) is a ~11 kDa protein. Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Sumoylation, the attachment of SUMO to target proteins, regulates multiple cellular events. _x005F_x000D_ _x005F_x000D_1. Johnson, ES. et al. (1997) The EMBO journal 16, 5509-19. PMID: 9312010 _x005F_x000D_ |
| Note | For research use only. |

SDS-PAGE for Recombinant SUMO tag Control Protein (N-terminal)
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