ZEBOV VP35 Recombinant Protein (N-His-SUMO) (VK661012)

Name: ZEBOV VP35 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: VK661012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	VK661012
Descripción	Recombinant ZEBOV VP24 Protein, N-His-SUMO (VK661012) expressed in E. coli, spanning Gly215-Ile340. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q05127
Longitud de proteína	Gly215-Ile340
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	26.21 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Polymerase cofactor VP35, Ebola VP35, eVP35, VP35
Fondo	Polymerase cofactor VP35 is a ~37 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1. 1. Basler, CF. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 12289-94. PMID: 11027311 2. van Tol, S. et al. (2022) PLoS pathogens 18, e1010532. PMID: 35533195 3. Enterlein, S. et al. (2006) Antimicrobial agents and chemotherapy 50, 984-93. PMID: 16495261 4. Brown, CS. et al. (2014) Journal of molecular biology 426, 2045-58. PMID: 24495995 5. Mühlberger, E. et al. (1999) Journal of virology 73, 2333-42. PMID: 9971816 6. Watanabe, R. et al. (2024) Cell 187, 5587-5603.e19. PMID: 39293445 7. Luthra, P. et al. (2015) Journal of virology 89, 5148-53. PMID: 25741013 8. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894 9. Yen, BC. et al. (2016) Journal of virology 90, 5108-5118. PMID: 26962215 10. Luthra, P. et al. (2013) Cell host & microbe 14, 74-84. PMID: 23870315
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant ZEBOV VP24 Protein, N-His-SUMO

Referencias

Datasheet

ZEBOV VP35 Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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