Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9) (VK661013)

Name: Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9)
Brand: abinScience
SKU: VK661013
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK661013

Description

Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9) (VK661013) is a human monoclonal antibody detecting VP35/Polymerase cofactor VP35 in ELISA, WB. Suitable for Zaire ebolavirus (ZEBOV) (Zaire Ebola virus).

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)

Applications

ELISA, WB

Host species

Human

Clonality

Monoclonal

Isotype

IgG

Clone ID

Target

VP35, Polymerase cofactor VP35, Ebola VP35, eVP35

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q05127

RRID

Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9) (abinScience Cat# VK661013, RRID:AB_3727248)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Polymerase cofactor VP35 is a ~37 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Basler, CF. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 12289-94. PMID: 11027311

2. van Tol, S. et al. (2022) PLoS pathogens 18, e1010532. PMID: 35533195

3. Enterlein, S. et al. (2006) Antimicrobial agents and chemotherapy 50, 984-93. PMID: 16495261

4. Brown, CS. et al. (2014) Journal of molecular biology 426, 2045-58. PMID: 24495995

5. Mühlberger, E. et al. (1999) Journal of virology 73, 2333-42. PMID: 9971816

6. Watanabe, R. et al. (2024) Cell 187, 5587-5603.e19. PMID: 39293445

7. Luthra, P. et al. (2015) Journal of virology 89, 5148-53. PMID: 25741013

8. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894

9. Yen, BC. et al. (2016) Journal of virology 90, 5108-5118. PMID: 26962215

10. Luthra, P. et al. (2013) Cell host & microbe 14, 74-84. PMID: 23870315

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-ZEBOV VP35/Polymerase cofactor VP35 Antibody (F9)

References

Datasheet

Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ZEBOV VP35/Polymerase cofactor VP35 Recombinant Antibody (F9) (VK661013)

Overview

Images

References

Datasheet

SDS