ZEBOV VP35 Recombinant Protein (N-His) (VK661022)

Name: ZEBOV VP35 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK661022
Price: 211 USD
Availability: InStock

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Numéro de catalogue	VK661022
Description	Recombinant ZEBOV VP35 Protein, N-His (VK661022) expressed in E. coli, spanning Gly215-Ile340. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q05127
Longueur de protéine	Gly215-Ile340
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	16.16 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Polymerase cofactor VP35, Ebola VP35, eVP35, VP35
Contexte	Polymerase cofactor VP35 is a ~37 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1. 1. Basler, CF. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 12289-94. PMID: 11027311 2. van Tol, S. et al. (2022) PLoS pathogens 18, e1010532. PMID: 35533195 3. Enterlein, S. et al. (2006) Antimicrobial agents and chemotherapy 50, 984-93. PMID: 16495261 4. Brown, CS. et al. (2014) Journal of molecular biology 426, 2045-58. PMID: 24495995 5. Mühlberger, E. et al. (1999) Journal of virology 73, 2333-42. PMID: 9971816 6. Watanabe, R. et al. (2024) Cell 187, 5587-5603.e19. PMID: 39293445 7. Luthra, P. et al. (2015) Journal of virology 89, 5148-53. PMID: 25741013 8. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894 9. Yen, BC. et al. (2016) Journal of virology 90, 5108-5118. PMID: 26962215 10. Luthra, P. et al. (2013) Cell host & microbe 14, 74-84. PMID: 23870315
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant ZEBOV VP35 Protein, N-His

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ZEBOV VP35 Recombinant Protein (N-His).pdf

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