Anti-Human IFN-alpha/beta R1 Reference Antibody (Anifrolumab, RUO) (HB823016)

Name: Anti-Human IFN-alpha/beta R1 Reference Antibody (Anifrolumab, RUO)
Brand: abinScience
SKU: HB823016
Price: 176 USD
Availability: InStock

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB823016
説明	Anifrolumab (HB823016) is a research-grade recombinant antibody targeting Interferon alpha/beta receptor 1. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
発現システム	Mammalian cells
テスト済みアプリケーション	WB
ターゲット	Interferon alpha/beta receptor 1, Cytokine receptor family 2 member 1, Cytokine receptor class-II member 1, CRF2-1, IFN-R-1, IFNAR1, IFNAR, IFN-alpha/beta receptor 1, Type I interferon receptor 1
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P17181
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	MDX-1333, MEDI-546, 1326232-46-5
背景	Interferon alpha/beta receptor 1 (IFNAR1) is a ~63 kDa protein. Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. 1. Russell-Harde, D. et al. (1999) Biochemical and biophysical research communications 255, 539-44. PMID: 10049744 2. Kumar, KG. et al. (2003) The EMBO journal 22, 5480-90. PMID: 14532120 3. Kumar, KG. et al. (2004) The Journal of biological chemistry 279, 46614-20. PMID: 15337770 4. Uzé, G. et al. (1990) Cell 60, 225-34. PMID: 2153461 5. Thomas, C. et al. (2011) Cell 146, 621-32. PMID: 21854986 6. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985 7. Hernandez, N. et al. (2019) The Journal of experimental medicine 216, 2057-2070. PMID: 31270247 8. Gothe, F. et al. (2022) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 74, 136-139. PMID: 33252644 9. Bastard, P. et al. (2022) The Journal of experimental medicine 219. PMID: 35442418 10. Abramovich, C. et al. (1994) The EMBO journal 13, 5871-7. PMID: 7813427
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

イメージ

Bioactivity

SEC-HPLC detection for Research Grade Anifrolumab.
SDS-PAGE

SDS-PAGE for Research Grade Anifrolumab.
Western blot

Western blot analysis was performed using anti-IFNAR1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IFNAR1 (Catalog No: HB823011), 50ng
Lane 2: recombinant human IFNAR1 (Catalog No: HB823011), 30ng
Lane 3: recombinant human IFNAR1 (Catalog No: HB823011), 20ng