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Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO) (JN878016)

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Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO)
Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO)
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Vista general
Número de catálogoJN878016
Reactividad de especiesClostridium perfringens
AplicacionesELISA, Bioactivity: FACS, Functional assay, Research in vivo
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1
ID de clon3a4D10
Sistema de expresión Mammalian cells
Objetivo Phospholipase C, PLC, 3.1.4.3, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, plc, cpa
Nivel de endotoxinas < 10 EU/mg
Pureza >95% purity as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso Q9RF12
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Phospholipase C (plcAlpha-toxin) is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing cell rupture. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage.

Referencias 1. Singh V, et al. Phospholipase C: underrated players in microbial infections. Front Cell Infect Microbiol. 2023 Apr 17;13:1089374. [JN878016]
Nota For research use only. Not suitable for clinical or therapeutic use.
Imágenes
  • Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Anti-Clostridium perfringens plcAlpha-toxin Antibody (3a4D10)

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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