Clostridium perfringens Alpha-toxin/PLC Recombinant Protein (N-His) (JN878032)

Name: Clostridium perfringens Alpha-toxin/PLC Recombinant Protein (N-His)
Brand: abinScience
SKU: JN878032
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN878032
Description	Recombinant Clostridium perfringens Alpha-toxin/PLC Protein, N-His (JN878032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9RF12
Protein length	Trp29-Lys398
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Clostridium perfringens
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Alpha-toxin, EC:3.1.4.3, Hemolysin, Lecithinase, PLC, Phosphatidylcholine cholinephosphohydrolase, Phospholipase C, cpa, plc
Background	Phospholipase C is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing cell rupture. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage.
Note	For research use only

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Datasheet

Clostridium perfringens Alpha-toxin/PLC Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Clostridium perfringens Alpha-toxin/PLC Recombinant Protein (N-His) (JN878032)

Overview

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References

Datasheet

SDS

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