Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO) (JN878016)

Name: Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO)
Brand: abinScience
SKU: JN878016
Price: 370 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN878016

Species reactivity

Clostridium perfringens

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Human

Isotype

IgG1

Clone ID

3a4D10

Expression system

Mammalian cells

Clonality

Monoclonal

Target

Phospholipase C, PLC, 3.1.4.3, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, plc, cpa

Endotoxin level

< 10 EU/mg

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9RF12

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phospholipase C (plcAlpha-toxin) is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing cell rupture. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage.

References

1. Singh V, et al. Phospholipase C: underrated players in microbial infections. Front Cell Infect Microbiol. 2023 Apr 17;13:1089374. [JN878016]

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Clostridium perfringens plcAlpha-toxin Antibody (3a4D10)

References

Datasheet

Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium perfringens plc Alpha-toxin Reference Antibody (3a4D10, RUO) (JN878016)

Overview

Images

References

Datasheet

SDS

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