

| Numéro de catalogue | JN878016 | ||||
|---|---|---|---|---|---|
| Reactivité des espèces | Clostridium perfringens | ||||
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | ||||
| Espèce hôte | Human | ||||
| Clonalité | Monoclonal | ||||
| Isotype | IgG1 | ||||
| ID de clone | 3a4D10 | ||||
| Système d'expression | Mammalian cells | ||||
| Cible | Phospholipase C, PLC, 3.1.4.3, Alpha-toxin, Hemolysin, Lecithinase, Phosphatidylcholine cholinephosphohydrolase, plc, cpa | ||||
| Niveau d'endotoxines | < 10 EU/mg | ||||
| Pureté | >95% purity as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Numéro d'accès | Q9RF12 | ||||
| Forme | Liquid | ||||
| Tampon de stockage | 0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
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| Informations d'utilisation du produit |
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| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| Contexte | Phospholipase C (plcAlpha-toxin) is a ~45 kDa protein. Bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture. Constitutes an essential virulence factor in gas gangrene. Binds to eukaryotic membranes where it hydrolyzes both phosphatidylcholine and sphingomyelin, causing cell rupture. The diacylglycerol produced can activate both the arachidonic acid pathway, leading to modulation of the inflammatory response cascade and thrombosis, and protein kinase C, leading to activation of eukaryotic phospholipases and further membrane damage. | ||||
| Reférences | 1. Singh V, et al. Phospholipase C: underrated players in microbial infections. Front Cell Infect Microbiol. 2023 Apr 17;13:1089374. [JN878016] | ||||
| Note | For research use only. Not suitable for clinical or therapeutic use. |

SDS-PAGE for Research Grade Anti-Clostridium perfringens plcAlpha-toxin Antibody (3a4D10)


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