Anti-Human FGFR2 Reference Antibody (GP369, RUO) (HB863036)

Name: Anti-Human FGFR2 Reference Antibody (GP369, RUO)
Brand: abinScience
SKU: HB863036
Price: 370 USD
Availability: InStock

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Numéro de catalogue	HB863036
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG
ID de clone	GP369
Activité biologique	GP369 (0.001-1000 nM, 2-5 days) can significantly inhibit the proliferation of various cells, including FDCP-1, SUM52PE and MCF7 cells [1]. GP369 (0.001-100 nM, 2 days) inhibits FGFR2 IIIb driven proliferation and has no effect on FGFR2 IIIc in FDCP-1 (IC 50 = 1.4 nM) cells [1]. GP369 (30 ug/mL, 3 days) inhibits proliferation induced by FGF7 (25 ng/mL) in MCF7 cells [1]. GP369 (10 ug/mL, 1-48 h) suppresses FGFR2 and ERK1/2 phosphorylation and downstream signaling in Ba/F3 and SNU-16 cells [1]. Cell Proliferation Assay [1] Cell Line: FDCP-1 cells Concentration: 0.001-100 nM Incubation Time: 2 days Result: Significantly inhibited FGFR2 IIIb driven proliferation, including FGFR2 IIIb WT, FGFR2 IIIb N550K and FGFR2 IIIb S252W. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 6, 24 and 48 h Result: Reduced the level of FGFR2 protein. Western Blot Analysis [1] Cell Line: Ba/F3 cells and SNU-16 cells Concentration: 5 ug/mL Incubation Time: Incubated for 1 h and then combinated with FGF7 for 15 min Result: Significantly inhibited FGF7 induced phosphorylation and downstream protein levels, including p-FGFR and p-ERK1/2. GP369 (2-20 mg/kg; i.p.; twice weekly; for 43 days) significantly inhibits tumor growth and reduces FGFR2 phosphorylation and protein levels in C.B-17 severe combined immunodeficient (SCID) mice bearing SNU-16 xenografts [1]. GP369 (20 mg/kg, i.p.; twice weekly; for 27 days) realizes tumor arrest and significantly reduces FGFR2 phosphorylation and protein levels in NCr nude mice bearing MFM-223 xenografts [1].
Système d'expression	Mammalian cells
Cible	K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P21802
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	GP369
Contexte	Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4.
Reférences	1. Bai A, et al. GP369, an FGFR2-IIIb-specific antibody, exhibits potent antitumor activity against human cancers driven by activated FGFR2 signaling. Cancer Res. 2010 Oct 1;70(19):7630-9. [HB863036]
Note	For research use only. Not suitable for clinical or therapeutic use.