Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO) (HB863056)

Name: Anti-Human FGFR2 Reference Antibody (HuGAL-FR21, RUO)
Brand: abinScience
SKU: HB863056
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HB863056
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	HuGAL-FR21
Biological activity	HuGAL-FR21 (0.001-1 ug/mL) can specifically bind to FGFR2IIIb (EC 50 approximately 20 pM) and its mutant S252W in SNU-16 cells [1]. HuGAL-FR21 (0.01-100 ug/mL) effectively blocks the binding of FGFR2IIIb (IC 50 approximately 1.5-3 ug/mL) to FGF2, FGF7, and FGF10 [1]. HuGAL-FR21 (10 ug/mL, 30 min) effectively blocks FGF7 induced phosphorylation and significantly inhibits FGF2 induced phosphorylation in SNU-16 cells [1]. HuGAL-FR21 (10 ug/mL, 2-24 h) downregulates FGFR2 protein levels in SNU-16 cells [1]. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 8 and 48 h Result: Reduced the level of FGFR2 protein by approximately 50%. HuGAL-FR21 (0.5-5 mg/kg; i.p.; twice weekly; for 25-30 days) effectively inhibits the growth of tumors and has no obvious toxicity in athymic nude mice bearing SNU-16 xenografts or OCUM-2M xenografts [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P21802
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	HuGAL-FR21
Background	Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4.
References	1. Zhao WM, et al. Monoclonal antibodies to fibroblast growth factor receptor 2 effectively inhibit growth of gastric tumor xenografts. Clin Cancer Res. 2010 Dec 1;16(23):5750-8. [HB863056]
Note	For research use only. Not suitable for clinical or therapeutic use.