Anti-Human FGFR2 Reference Antibody (GP369, RUO) (HB863036)

Name: Anti-Human FGFR2 Reference Antibody (GP369, RUO)
Brand: abinScience
SKU: HB863036
Price: 370 USD
Availability: InStock

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概要
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参考文献
データシート
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概要

カタログ番号	HB863036
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	GP369
生物学的活性	GP369 (0.001-1000 nM, 2-5 days) can significantly inhibit the proliferation of various cells, including FDCP-1, SUM52PE and MCF7 cells [1]. GP369 (0.001-100 nM, 2 days) inhibits FGFR2 IIIb driven proliferation and has no effect on FGFR2 IIIc in FDCP-1 (IC 50 = 1.4 nM) cells [1]. GP369 (30 ug/mL, 3 days) inhibits proliferation induced by FGF7 (25 ng/mL) in MCF7 cells [1]. GP369 (10 ug/mL, 1-48 h) suppresses FGFR2 and ERK1/2 phosphorylation and downstream signaling in Ba/F3 and SNU-16 cells [1]. Cell Proliferation Assay [1] Cell Line: FDCP-1 cells Concentration: 0.001-100 nM Incubation Time: 2 days Result: Significantly inhibited FGFR2 IIIb driven proliferation, including FGFR2 IIIb WT, FGFR2 IIIb N550K and FGFR2 IIIb S252W. Western Blot Analysis [1] Cell Line: SNU-16 cells Concentration: 10 ug/mL Incubation Time: 2, 6, 24 and 48 h Result: Reduced the level of FGFR2 protein. Western Blot Analysis [1] Cell Line: Ba/F3 cells and SNU-16 cells Concentration: 5 ug/mL Incubation Time: Incubated for 1 h and then combinated with FGF7 for 15 min Result: Significantly inhibited FGF7 induced phosphorylation and downstream protein levels, including p-FGFR and p-ERK1/2. GP369 (2-20 mg/kg; i.p.; twice weekly; for 43 days) significantly inhibits tumor growth and reduces FGFR2 phosphorylation and protein levels in C.B-17 severe combined immunodeficient (SCID) mice bearing SNU-16 xenografts [1]. GP369 (20 mg/kg, i.p.; twice weekly; for 27 days) realizes tumor arrest and significantly reduces FGFR2 phosphorylation and protein levels in NCr nude mice bearing MFM-223 xenografts [1].
発現システム	Mammalian cells
ターゲット	K-sam, KSAM, CD332, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR-2, FGFR2, KGFR, BEK
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P21802
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	GP369
背景	Fibroblast growth factor receptor 2 (CD332/FGFR2) is a ~92 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4.
参考文献	1. Bai A, et al. GP369, an FGFR2-IIIb-specific antibody, exhibits potent antitumor activity against human cancers driven by activated FGFR2 signaling. Cancer Res. 2010 Oct 1;70(19):7630-9. [HB863036]
注意事項	For research use only. Not suitable for clinical or therapeutic use.