Anti-Human GFR alpha-like Reference Antibody (NGM120, RUO) (HS720016)

Name: Anti-Human GFR alpha-like Reference Antibody (NGM120, RUO)
Brand: abinScience
SKU: HS720016
Price: 176 USD
Availability: InStock

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概要

カタログ番号	HS720016
説明	Anti-Human GFRAL (NGM120) (HS720016) is a research-grade recombinant antibody targeting C6orf144. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	NGM120
発現システム	Mammalian cells
ターゲット	C6orf144, GDNF family receptor alpha-like, GFRAL
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	Q6UXV0
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	NGM120
背景	GDNF family receptor alpha-like (GFRAL) is a ~44 kDa protein. Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. 1. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097 2. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098 3. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099 4. Hsu, JY. et al. (2017) Nature 550, 255-259. PMID: 28953886 5. Benichou, O. et al. (2023) Cell metabolism 35, 274-286.e10. PMID: 36630958 7. Li, J. et al. (2019) eLife 8. PMID: 31535977
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 (LALA) Recombinant Isotype Control Antibody (HyHEL-10) (HP688107)

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SDS-PAGE

SDS-PAGE for Research Grade Anti-Human GFRAL (NGM120).
Western blot

Western blot analysis was performed using anti-GFRAL monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human GFRAL (Catalog No: HS720011), 50ng
Lane 2: recombinant human GFRAL (Catalog No: HS720011), 30ng

参考文献

データシート

Anti-Human GFR alpha-like Reference Antibody (NGM120, RUO).pdf

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Anti-Human GFR alpha-like Reference Antibody (NGM120, RUO) (HS720016)

概要

イメージ

参考文献

データシート

SDS

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