Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO) (HY196056)

Name: Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO)
Brand: abinScience
SKU: HY196056
Price: 370 USD
Availability: InStock

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Numéro de catalogue	HY196056
Reactivité des espèces	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG
ID de clone	CE-355621
Activité biologique	CE-355621 (0.01-100 nM, 4 h) can efficiently block the binding of c-Met to HGF and induce c-Met degradation in A549 cells [1]. CE-355621 (0.01 nM-10 nM, 24 h) significantly inhibits c-Met activation in U87MG cells [1]. CE-355621 (0.01-10 ug/mL, 48 h) can induce c-Met degradation in U87MG cells [1]. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01 nM-10 nM Incubation Time: 24 h Result: Significantly inhibited activation of c-Met. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01-10 ug/mL Incubation Time: 48 h Result: Reduced the level of c-Met. CE-355621 (25-200 ug, i.p., on day 0, 2, 3, 6 and 8) significantly delays tumor growth in female CD-1 nu/nu mice bearing U87MG xenografts [1]. CE-355621 (400 ug, i.p., on day 7 and 14) significantly inhibits tumor growth in female CD-1 nu/nu mice bearing GTL-16 xenografts [1]. CE-355621 (200 ug, i.p., single dose, on day 7) can effectively inhibit tumor volume growth and uptake of 18 F-FDG in female nude (nu/nu) mice bearing U87MG xenografts [2].
Système d'expression	Mammalian cells
Cible	Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
Niveau d'endotoxines	< 10 EU/mg
Pureté	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P08581
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CE-355621, CE355621, CE355621
Contexte	Hepatocyte growth factor receptor (MET/HGFR) is a ~155 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. MET is the therapeutic target of amivantamab (Rybrevant).
Reférences	1. Michaud NR, et al. Biochemical and pharmacological characterization of human c-Met neutralizing monoclonal antibody CE-355621. MAbs. 2012 Nov-Dec;4(6):710-23. [HY196056] 2. Tseng JR, et al. Preclinical efficacy of the c-Met inhibitor CE-355621 in a U87 MG mouse xenograft model evaluated by 18F-FDG small-animal PET. J Nucl Med. 2008 Jan;49(1):129-134. [HY196056]
Note	For research use only. Not suitable for clinical or therapeutic use.