Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO) (HY196056)

Name: Anti-Human HGFR/c-MET Reference Antibody (CE-355621, RUO)
Brand: abinScience
SKU: HY196056
Price: 370 USD
Availability: InStock

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概要
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参考文献
データシート
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概要

カタログ番号	HY196056
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG
クローンID	CE-355621
生物学的活性	CE-355621 (0.01-100 nM, 4 h) can efficiently block the binding of c-Met to HGF and induce c-Met degradation in A549 cells [1]. CE-355621 (0.01 nM-10 nM, 24 h) significantly inhibits c-Met activation in U87MG cells [1]. CE-355621 (0.01-10 ug/mL, 48 h) can induce c-Met degradation in U87MG cells [1]. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01 nM-10 nM Incubation Time: 24 h Result: Significantly inhibited activation of c-Met. Western Blot Analysis [1] Cell Line: U87MG cells Concentration: 0.01-10 ug/mL Incubation Time: 48 h Result: Reduced the level of c-Met. CE-355621 (25-200 ug, i.p., on day 0, 2, 3, 6 and 8) significantly delays tumor growth in female CD-1 nu/nu mice bearing U87MG xenografts [1]. CE-355621 (400 ug, i.p., on day 7 and 14) significantly inhibits tumor growth in female CD-1 nu/nu mice bearing GTL-16 xenografts [1]. CE-355621 (200 ug, i.p., single dose, on day 7) can effectively inhibit tumor volume growth and uptake of 18 F-FDG in female nude (nu/nu) mice bearing U87MG xenografts [2].
発現システム	Mammalian cells
ターゲット	Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P08581
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	CE-355621, CE355621, CE355621
背景	Hepatocyte growth factor receptor (MET/HGFR) is a ~155 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. MET is the therapeutic target of amivantamab (Rybrevant).
参考文献	1. Michaud NR, et al. Biochemical and pharmacological characterization of human c-Met neutralizing monoclonal antibody CE-355621. MAbs. 2012 Nov-Dec;4(6):710-23. [HY196056] 2. Tseng JR, et al. Preclinical efficacy of the c-Met inhibitor CE-355621 in a U87 MG mouse xenograft model evaluated by 18F-FDG small-animal PET. J Nucl Med. 2008 Jan;49(1):129-134. [HY196056]
注意事項	For research use only. Not suitable for clinical or therapeutic use.