中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HY554016 |
|---|---|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1-kappa |
| 発現システム | Mammalian cells |
| ターゲット | GCP/IL-8 protein II, MDNCF, IL8/NAP1 form III, IL-8(1-77), Neutrophil-activating factor, GCP/IL-8 protein I, GCP/IL-8 protein VI, Interleukin-8, Chemokine (C-X-C motif) ligand 8, Neutrophil-activating protein 1, Monocyte-derived neutrophil chemotactic factor, (Ser-IL-8)72, MDNCF-b, LYNAP, GCP/IL-8 protein V, IL8/NAP1 form IV, MONAP, IL8/NAP1 form V, IL8/NAP1 form VI, NAP-1, Granulocyte chemotactic protein 1, GCP/IL-8 protein III, IL8/NAP1 form II, IL8, IL-8, CXCL8, IL8/NAP1 form I, (Ala-IL-8)77, Protein 3-10C, GCP/IL-8 protein IV, Emoctakin, GCP-1, C-X-C motif chemokine 8, NAF, T-cell chemotactic factor, MDNCF-c, Monocyte-derived neutrophil-activating peptide, Lymphocyte-derived neutrophil-activating factor |
| エンドトキシンレベル | < 10 EU/mg |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P10145 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HuMab10F8, HuMax-IL8, MDX-018, formerlyHuMax-Inflam |
| 背景 | Interleukin-8 (IL8/CXCL8) is a ~11 kDa protein. Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways. 1. Zinkernagel, AS. et al. (2008) Cell host & microbe 4, 170-8. PMID: 18692776 2. Hammond, ME. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 155, 1428-33. PMID: 7636208 3. Van Damme, J. et al. (1990) European journal of immunology 20, 2113-8. PMID: 2145175 4. Jacobi, HH. et al. (1998) International archives of allergy and immunology 116, 53-9. PMID: 9623510 5. Holmes, WE. et al. (1991) Science (New York, N.Y.) 253, 1278-80. PMID: 1840701 6. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716 7. Takami, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 4559-66. PMID: 11971003 8. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698 |
| 参考文献 | 1. Edward John BERTACCINI, et al. Novel methods, compounds, and compositions for anesthesia. WO2016061538A1. 2018-12-05 [HY554016] |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SDS-PAGE for Research Grade HuMax-IL8.
SEC-HPLC detection for Research Grade HuMax-IL8.
Detects Human IL8/CXCL8 in indirect ELISAs.
Western blot analysis was performed using anti-IL8/CXCL8 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL8/CXCL8 (Catalog No: HY554011), 50ng
Lane 2: recombinant human IL8/CXCL8 (Catalog No: HY554011), 30ng
Lane 3: recombinant human IL8/CXCL8 (Catalog No: HY554011), 20ng
Lane 4: recombinant human IL8/CXCL8 (Catalog No: HY554011), 10ng
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
