ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species
Human
Isotype
IgG2-kappa
Expression system
Mammalian Cells
Clonality
Monoclonal
Target
GCP/IL-8 protein II, MDNCF, IL8/NAP1 form III, IL-8(1-77), Neutrophil-activating factor, GCP/IL-8 protein I, GCP/IL-8 protein VI, Interleukin-8, Chemokine (C-X-C motif) ligand 8, Neutrophil-activating protein 1, Monocyte-derived neutrophil chemotactic factor, (Ser-IL-8)72, MDNCF-b, LYNAP, GCP/IL-8 protein V, IL8/NAP1 form IV, MONAP, IL8/NAP1 form V, IL8/NAP1 form VI, NAP-1, Granulocyte chemotactic protein 1, GCP/IL-8 protein III, IL8/NAP1 form II, IL8, IL-8, CXCL8, IL8/NAP1 form I, (Ala-IL-8)77, Protein 3-10C, GCP/IL-8 protein IV, Emoctakin, GCP-1, C-X-C motif chemokine 8, NAF, T-cell chemotactic factor, MDNCF-c, Monocyte-derived neutrophil-activating peptide, Lymphocyte-derived neutrophil-activating factor
Endotoxin level
Please contact the lab for this information.
Purity
>95% purity as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P10145
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
ABX-IL8
Background
Interleukin-8 (IL8/CXCL8) is a ~11 kDa protein. Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways.
2. Hammond, ME. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 155, 1428-33. PMID: 7636208
3. Van Damme, J. et al. (1990) European journal of immunology 20, 2113-8. PMID: 2145175
4. Jacobi, HH. et al. (1998) International archives of allergy and immunology 116, 53-9. PMID: 9623510
5. Holmes, WE. et al. (1991) Science (New York, N.Y.) 253, 1278-80. PMID: 1840701
6. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716
7. Takami, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 4559-66. PMID: 11971003
8. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
SEC-HPLC
SEC-HPLC detection for Research Grade ABX-IL8.
Bioactivity
Detects IL8/CXCL8 in indirect ELISAs.
SDS-PAGE
SDS-PAGE for Research Grade ABX-IL8
Western blot
Western blot analysis was performed using anti-IL8/CXCL8 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human IL8/CXCL8 (Catalog No: HY554011) Lane 2: negative control
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