









| Número de catálogo | HF879036 |
|---|---|
| Descripción |
Infliximab (HF879036) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| Reactividad de especies | Human |
| Aplicaciones | ELISA, Bioactivity: FCM, Functional assay, Research in vivo |
| Especie huésped | Chimeric |
| Clonalidad | Monoclonal |
| Isotipo | IgG1, kappa |
| Aplicaciones probadas | FCM |
| Objetivo | C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form |
| Nivel de endotoxinas | < 10 EU/mg |
| Pureza | >95% as determined by SDS-PAGE. |
| Purificación | Protein A/G purified from cell culture supernatant. |
| Número de acceso | P01375 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Nombres alternativos | TA-650, cA2, 170277-31-3 |
| Fondo | Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research. |
| Nota | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |

SEC-HPLC detection for Research Grade Infliximab.

Detects Human TNFa/TNF-alpha in indirect ELISAs.

SDS-PAGE for Anti-Human TNF-alpha Reference Antibody (Infliximab, RUO)

Flow-cytometry PE using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human TNFa monoclonal antibody (Catalog: HF879036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF879036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.




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